Fertility Decline Research Articles

Star1 gene mutation reveals the essentiality of 11-ketotestosterone and glucocorticoids for male fertility in Nile Tilapia (Oreochromis niloticus)

Steroidogenic acute regulatory protein (Star) plays an essential role in the biosynthesis of corticosteroids and sex steroids by mediating the transport of cholesterol from the outer to the inner membrane of mitochondria. Two duplicated Star genes, namely star1 and star2, have been identified in non-mammalian vertebrates. To investigate the roles of star genes in fish steriodogenesis, we generated two mutation lines of star1−/− and star1−/−/star2−/− in Nile tilapia (Oreochromis niloticus). Previous studies revealed that deficiency of star2 gene caused delayed spermatogenesis, sperm apoptosis and sterility in male tilapia. Our present data revealed that mutation of star genes impaired male fertility. Disordered seminiferous lobules and spermatic duct obstruction were found in the testis of both types of mutants. Moreover, significant decline in semen volume, sperm abnormality and impaired fertility were also detected in star1−/− and star1−/−/star2−/− males. In star1−/− male fish, lipid accumulation, up-regulation of steroidogenic enzymes, and significant decline of androgens were found. Additionally, hyperplasic interrenal cells, elevated steroidogenic gene expression level and decline of serum glucocorticoids were detected in star1 mutants. Intriguingly, either 11-KT or cortisol supplementation successfully rescued the impaired fertility of the star1−/− mutants. Taken together, these results further indicate that Star1 might play critical roles in the production of both 11-KT and glucocorticoids, which are indispensable for the maintenance of male fertility in fish.

Learning from Ethiopia’s success in reducing maternal and neonatal mortality through a health systems lens

BackgroundThis study aimed to enhance insights into the key characteristics of maternal and neonatal mortality declines in Ethiopia, conducted as part of a seven-country study on Maternal and Newborn Health...

Microfoundations of the weakening educational gradient in fertility

The disappearance of the social gradient in fertility represents a paradigm shift that has called into question the validity of theories that predicted a decline in fertility with increased access to education and resources. Emerging theories have tried to explain this trend by highlighting a potential change in the fertility preferences of more educated couples. In this paper we add additional elements to this explanation. Using a computational modelling approach, we show that it is still possible to simulate the weakening social gradient in fertility, in the context of steady declines in family size preferences. Our results show that one of the key drivers of the change in the education–fertility relationship can be found in the transition to an increasingly regulated fertility regime. As the share of unplanned births decreases over time, the negative association between education and fertility weakens and the mechanisms that positively connect educational attainment with desired fertility become dominant.

The Reproductive Journeys of French Women over 40 seeking Assisted Reproductive Technology Treatments in Spain

Fertility decline is a complex phenomenon resulting from converging social and cultural changes that are governed through politics. As it has been discussed in many studies, the European’s low fertility rate goes hand in hand with the trend of maternity postponement. Although in many European countries over the last decades reproduction is envisaged as an individual choice that can be made at older ages, having a child after a certain age can be impossible -either “naturally” or using assisted reproductive technology- depending on the medical and legislative possibilities and limits of the country in which people live. In the extremely diverse European reproscape, reproductive legislations have forced but also allowed many people to seek reproductive treatments outside their home countries.Spain is a leading destination in Europe for cross border reproductive travel and, of the foreigners it receives, the French are the largest group. Despite having a history of strong pro-natalist policies, France has been one of the strictest European countries regarding access to medically assisted procreation. Until 2022, only heterosexual couples in which women were under 43 years of age could access treatments. Despite the recent opening of access to “all women”, including single women and same-sex female couples, women over 43 years of age were once again excluded from the new legal framework and therefore remain condemned to travel abroad to access reproductive treatments. In this article, we analyze the experience of French women over 40 who cross the Spanish border to access reproductive treatments in order to fulfill their desire to have children. Through ethnographic data emerging from six years of participant observation and in-depth interviews with 15 women, we explore why they remain excluded from the French system of reproductive governance and the obstacles they face during their reproductive journey.

Glyphosate presence in human sperm: First report and positive correlation with oxidative stress in an infertile French population

Environmental exposure to endocrine disruptors, such as pesticides, could contribute to a decline of human fertility. Glyphosate (GLY) is the main component of Glyphosate Based Herbicides (GBHs), which are the most commonly herbicides used in the world. Various animal model studies demonstrated its reprotoxicity. In Europe, GLY authorization in agriculture has been extended until 2034. Meanwhile the toxicity of GLY in humans is still in debate. The aims of our study were firstly to analyse the concentration of GLY and its main metabolite, amino-methyl-phosphonic acid (AMPA) by LC/MS-MS in the seminal and blood plasma in an infertile French men population (n=128). We secondly determined Total Antioxidant Status (TAS) and Total Oxidant Status (TOS) using commercial colorimetric kits and some oxidative stress biomarkers including malondialdehyde (MDA) and 8-hydroxy-2′-deoxyguanosine (8-OHdG) by ELISA assays. We next analysed potential correlations between GLY and oxidative stress biomarkers concentration and sperm parameters (sperm concentration, progressive speed, anormal forms). Here, we detected for the first time GLY in the human seminal plasma in significant proportions and we showed that its concentration was four times higher than those observed in blood plasma. At the opposite, AMPA was undetectable. We also observed a strong positive correlation between plasma blood GLY concentrations and plasma seminal GLY and 8-OHdG concentrations, the latter reflecting DNA impact. In addition, TOS, Oxidative Stress Index (OSI) (TOS/TAS), MDA blood and seminal plasma concentrations were significantly higher in men with glyphosate in blood and seminal plasma, respectively. Taken together, our results suggest a negative impact of GLY on the human reproductive health and possibly on his progeny. A precaution principle should be applied at the time of the actual discussion of GLY and GBHs formulants uses in Europe by the authorities.

Gallic acid supplementation partially ameliorates reproductive aging in rooster breeders by improving semen quality, sperm kinetics, hormones, and antioxidant status

Aging leads to decreased fertility in roosters, which is likely due to increased oxidative stress. This study evaluated the antioxidant effects of gallic acid (GA) supplementation on sperm quality and fertility of aged roosters. This study evaluated whether gallic acid (GA) supplementation can mitigate age-related fertility decline. Roosters were randomly assigned to: control, 100 mg/kg GA, or 200 mg/kg GA. Semen parameters, sperm kinetics, hormone levels, fertility rate, and hatchability were assessed. GA increased semen concentration, membrane integrity and viability while decreasing defects versus control (P<0.01). Testosterone was higher in GA groups (P<0.01) without affecting gonadotropins. Furthermore, 200 mg/kg GA optimized motility, velocity, linearity, and beat cross frequency versus control and 100 mg/kg GA (P<0.01). Fertility and hatchability were higher in both GA groups. In conclusion, GA supplementation in aged roosters improves sperm quality, antioxidant status, testosterone, and fertility outcomes, likely by mitigating oxidative stress. The 200 mg/kg dose elicited optimal effects on motion parameters.

Understanding the interactions of genotype with environment and management (G×E×M) to maize productivity in Conservation Agriculture systems of Malawi.

Climatic variability and soil fertility decline present a fundamental challenge for smallholder farmers to determine the optimum management practices in the production of maize. Optimizing genotype (G) and management (M) of maize under different environmental conditions (E) and their interactions are essential for enhancing maize productivity in the smallholder sector of Malawi where maize is the main staple food. Here, we evaluated over seven seasons, the performance of four commercial maize genotypes [including hybrids and one open pollinated variety (OPV)] managed under different Conservation Agriculture (CA) and conventional practices (CP) across on-farm communities of central and southern Malawi. Our results revealed significant G×E and E×M interactions and showed that hybrids such as DKC 80-53 and PAN 53 outyielded the other hybrid and the OPV in most of the environments while the OPV ZM523 had greater yields in environments with above-average rainfall and shorter in-season dry spells. These environments received a maximum of 1250 mm to 1500 mm of rainfall and yet the long-term averages were 855 mm and 1248 mm, respectively. Despite yielding lower, the OPV ZM523 also exhibited higher yield stability across environments compared to the hybrid MH 30, possibly due to its resilience to drought, heat stress, and low soil fertility conditions which are often prevalent in the target communities. Conservation Agriculture-based practices outyielded CP across the genotypes and environments. However, amongst the CA-based systems, intercropping of maize with pigeonpea [Cajanus cajan (L.) Millsp] and cowpea (Vigna unguiculata Walp.) performed less than monocropping maize and then rotating it with a legume probably due to competition for moisture between the main and the companion crops in the intercrop. The key findings of this study suggest the need to optimize varietal and management options for particular environments to maximize maize productivity in Malawi. This means that smallholder farmers in Malawi should adopt hybrids and CA-based systems for enhanced yields but could also consider OPVs where the climate is highly variable. Further rigorous analysis that includes more abiotic stress factors is recommended for a better understanding of yield response.

Influence of substance use on male reproductive health and offspring outcomes.

The prevalence of substance use globally is rising and is highest among men of reproductive age. In Africa, and South and Central America, cannabis use disorder is most prevalent and in Eastern and South-Eastern Europe, Central America, Canada and the USA, opioid use disorder predominates. Substance use might be contributing to the ongoing global decline in male fertility, and emerging evidence has linked paternal substance use with short-term and long-term adverse effects on offspring development and outcomes. This trend is concerning given that substance use is increasing, including during the COVID-19 pandemic. Preclinical studies have shown that male preconception substance use can influence offspring brain development and neurobehaviour through epigenetic mechanisms. Additionally, human studies investigating paternal health behaviours during the prenatal period suggest that paternal tobacco, opioid, cannabis and alcohol use is associated with reduced offspring mental health, in particular hyperactivity and attention-deficit hyperactivity disorder. The potential effects of paternal substance use are areas in which to focus public health efforts and health-care provider counselling of couples or individuals interested in conceiving.

What is driving the global decline of human fertility? Need for a multidisciplinary approach to the underlying mechanisms.

An intense period of human population expansion over the past 250 years is about to cease. Total fertility rates are falling dramatically all over the world such that highly industrialized nations, including China and the tiger economies of SE Asia, will see their populations decline significantly in the coming decades. The socioeconomic, geopolitical and environmental ramifications of this change are considerable and invite a multidisciplinary consideration of the underlying mechanisms. In the short-term, socioeconomic factors, particularly urbanization and delayed childbearing are powerful drivers of reduced fertility. In parallel, lifestyle factors such as obesity and the presence of numerous reproductive toxicants in the environment, including air-borne pollutants, nanoplastics and electromagnetic radiation, are seriously compromising reproductive health. In the longer term, it is hypothesized that the reduction in family size that accompanies the demographic transition will decrease selection pressure on high fertility genes leading to a progressive loss of human fecundity. Paradoxically, the uptake of assisted reproductive technologies at scale, may also contribute to such fecundity loss by encouraging the retention of poor fertility genotypes within the population. Since the decline in fertility rate that accompanies the demographic transition appears to be ubiquitous, the public health implications for our species are potentially devastating.

House prices and fertility: Can the Dutch housing crisis explain the post‐2010 fertility decline?

AbstractFertility has declined in many rich societies after 2010. However, the factors that explain this fertility decline remain poorly understood. In particular, little is known about how changes on the housing market contributed to the fertility decline. This study examines the links between house prices and fertility in the Netherlands, a country where house prices have risen dramatically in the past decade. Regional (NUTS 3) data for the period 1995–2022 are analysed using within‐between models. The results for the within‐effect of house prices show that after 2010, fertility decreased (more) in regions where house prices increased (more), suggesting that the housing crisis in the Netherlands can explain a substantial part of the post‐2010 fertility decline. Before 2010, however, increases in regional house prices were associated with small increases in fertility, showing that the direction of the within‐effect of house prices depends on the time period that is studied. Finally, differences in house prices between regions are not consistently associated with fertility.

Moving up and down the urban hierarchy: Age‐specific internal migration patterns in Japan based on the 2020 census

AbstractThis study aimed to understand the age‐specific internal migration patterns in Japan where more than a quarter of the population is aged ≥65 years and the national population is on the decline, focusing on the urban hierarchy. Demographic efficiency, which is the ratio of net to gross migration, was calculated for each migration flow between the levels of urban hierarchy in Japan based on the 2020 census. The results showed that the overall upward migration, which is a movement from lower to higher levels in the urban hierarchy, was dominant. A variation in migration patterns by age confirmed that young adults, especially those aged 20–24 years, moved up the urban hierarchy unidirectionally, while people of preretirement and retirement age moved down. The older adults (aged 75 and over) were inclined to move up. Regarding sex‐based differences, the demographic efficiency of women was higher than that of men. Additionally, the upward movement of women was predominant in almost all age groups. These findings suggest the possibility that aggregated migration behaviours may vary by sex, which has received little attention in previous migration studies focusing on urban hierarchies in other countries. Furthermore, this study discussed policy implications based on the observed patterns, such as the impacts of older adults' migration on social security at each hierarchy level and a possible relationship between women's upward migration patterns and future fertility decline.

Heat stress impairs centromere structure and segregation of meiotic chromosomes in Arabidopsis

Heat stress is a major threat to global crop production, and understanding its impact on plant fertility is crucial for developing climate-resilient crops. Despite the known negative effects of heat stress on plant reproduction, the underlying molecular mechanisms remain poorly understood. Here, we investigated the impact of elevated temperature on centromere structure and chromosome segregation during meiosis in Arabidopsis thaliana. Consistent with previous studies, heat stress leads to a decline in fertility and micronuclei formation in pollen mother cells. Our results reveal that elevated temperature causes a decrease in the amount of centromeric histone and the kinetochore protein BMF1 at meiotic centromeres with increasing temperature. Furthermore, we show that heat stress increases the duration of meiotic divisions and prolongs the activity of the spindle assembly checkpoint during meiosis I, indicating an impaired efficiency of the kinetochore attachments to spindle microtubules. Our analysis of mutants with reduced levels of centromeric histone suggests that weakened centromeres sensitize plants to elevated temperature, resulting in meiotic defects and reduced fertility even at moderate temperatures. These results indicate that the structure and functionality of meiotic centromeres in Arabidopsis are highly sensitive to heat stress, and suggest that centromeres and kinetochores may represent a critical bottleneck in plant adaptation to increasing temperatures.

Heat stress impairs centromere structure and segregation of meiotic chromosomes in Arabidopsis.

Heat stress is a major threat to global crop production, and understanding its impact on plant fertility is crucial for developing climate-resilient crops. Despite the known negative effects of heat stress on plant reproduction, the underlying molecular mechanisms remain poorly understood. Here, we investigated the impact of elevated temperature on centromere structure and chromosome segregation during meiosis in Arabidopsis thaliana. Consistent with previous studies, heat stress leads to a decline in fertility and micronuclei formation in pollen mother cells. Our results reveal that elevated temperature causes a decrease in the amount of centromeric histone and the kinetochore protein BMF1 at meiotic centromeres with increasing temperature. Furthermore, we show that heat stress increases the duration of meiotic divisions and prolongs the activity of the spindle assembly checkpoint during meiosis I, indicating an impaired efficiency of the kinetochore attachments to spindle microtubules. Our analysis of mutants with reduced levels of centromeric histone suggests that weakened centromeres sensitize plants to elevated temperature, resulting in meiotic defects and reduced fertility even at moderate temperatures. These results indicate that the structure and functionality of meiotic centromeres in Arabidopsis are highly sensitive to heat stress, and suggest that centromeres and kinetochores may represent a critical bottleneck in plant adaptation to increasing temperatures.

Meiotic errors in oocytes of young and advanced maternal age women: the U-curve of fertility

Italy is currently one of the countries most affected by declining birth rates, a negative trend mainly determined by several socio-economic factors that lead women to postpone pregnancy. The use of in vitro fertilization techniques cannot counteract the natural decline in fertility that accompanies advancing maternal age. This decline is associated with an increase in chromosomal aneuploidy in oocytes that, if fertilized, could result in aneuploid embryos that are often miscarried. Here, we discuss the “molecular clock hypothesis” proposed by Hoffmann’s lab, which suggests a U-shaped trend of female fertility. The high rates of chromosomal aneuploidy observed at the two “ends of the curve” may represent an evolutionary mechanism of protection that exists to balance the risks associated with pregnancy. This hypothesis lays the foundations for debating the idea that very young women are free from the risk of aneuploidy, and also suggests the existence of a safety mechanism that “protects” against possibly complicated pregnancies both after the age of 40 years and in very young women. The U-shape hypothesis described in humans will be discussed in a comparison with the fertility trends of other mammalian species. KEY WORDS: Fertility, aneuploidy, advanced maternal age, IVF

Assessing soil erosion and farmers’ decision of reducing erosion for sustainable soil and water conservation in Burji woreda, southern Ethiopia

Inadequate conservation practice affects the sustainable production of agricultural watersheds due to erosion and fertility decline. Understanding soil erosion and implementing site-specific conservation practice could enhance agriculture-based rural development. The study was aimed to document soil erosion problem and soil and water conservation effort. The specific objectives of this study were to assess soil erosion severity, practices to reduce erosion, and determinants of the decision to reduce erosion. Data were collected by interviewing 198 farm household heads, undertaking four focus group discussions, and assessing rill erosion in 10 farm fields in Morayo and Wacho sub-watersheds of southern Ethiopia. Descriptive statistics and binary logit model were applied to analyze the data. Results indicated that many of the farm households, 63% in Morayo and 83% in the Wacho sub-watershed, perceived moderate to severe soil erosion, which is characterized by big rills and small gullies on the farmlands. Rill densities of 231.4 m ha−1 and 84.1 m ha−1 in the Morayo and Wacho sub-watersheds were observed, respectively. The estimated annual soil loss due to rills was 61.2 and 23.4 Mg ha−1 in the Morayo and Wacho sub-watersheds, respectively. The soil erosion from rills alone exceeds the expected tolerable soil erosion (11 tons ha−1 year−1). Due to erosion, about 90% of farmers perceived farmland degradation as described by a progressive decline in crop yield. Farmers used to practice traditional techniques to reduce erosion and government introduced conservation measures such as soil and stone bunds. However, many farmers did not use well-promoted conservation measures such as bunds, which could have negative impact on long-term erosion control effort and sustainable implementation of the conservation options. Among the assessed explanatory variables, educational level, farm distance from home, slope of the cultivated land, and frequency of extension contact were significantly affected (p < 0.05) farmers’ sustainable use of conservation measures. Development planners and policy makers are advised to consider site-specific and innovative approaches to implement conservation measures in sustainable approach in the smallholder crop-livestock mixed agriculture system.

Vulnerability and resilience in the face of climate changes in Senegal's drylands: measurement at the household level and determinant assessment

The resilience capacity of smallholder households is one of the main drivers of their ability to continue to farm and make investments in the fragile dryland regions. This paper aims to assess the resilience profile of smallholder farmers in the face of climate change and the factors influencing it in three dryland sub-regions of Senegal, namely, Louga, Kaffrine, and Thies. We developed a composite index of climate resilience (CICR) using data on farmers' perceptions of climate variability and their perceived ability to withstand, adapt, and bounce back in the event of climatic shocks. Drought, strong winds, and soil fertility decline because of climate change emerged as the main climate hazards impacting smallholder farming systems. The CICR value ranged from −2 for the most vulnerable households to +2 for the most resilient households. On average, all the households were found to be vulnerable, with an average CICR value of −0.2. The LOUGA region was the most vulnerable, with an average CICR value of −0.36, followed by THIES (-0.2). The KAFFRINE region was relatively less vulnerable, with a CICR value of −0.1. Ordered logit model estimates show that the chances of improving CICR decrease with the increase of the household head's age until 59 years. Access to training on climate-smart agricultural (CSA) practices and climate information appeared to have the potential to increase by 171% the chance of the household improving its resilience status. Analysis also shows that one more woman working off-farm or in-home gardening has the potential to multiply by four times the chances of households being more resilient. This highlights the importance of empowering women to enhance household resilience to climate change. The off-farm revenue increased the chance to improve the resilience status of the farm household by 62% and the receipt of transfer revenue by 50%. This study provides a robust method for quantifying resilience or wellbeing and its drivers and enriches our understanding of the resilience ability of farmers to climate change in a West African context. It can be useful in designing effective adaptation interventions and improving the overall wellbeing of smallholder farmers.

Fertility Knowledge and Educational Experiences of Graduating Medical Students: A Multi-Institution Survey.

Objective: Our goal was to assess the fertility knowledge and educational experiences of graduating U.S. medical students to evaluate areas of improvement for future educational interventions. Design: Web-based cross-sectional survey. Subjects: Medical students graduating in 2023 from the University of Miami Miller School of Medicine (SOM), New York University (NYU) Grossman SOM, Wayne State University SOM, Perelman SOM at the University of Pennsylvania, and Mayo Clinic Alix SOM. Main Outcome Measures: Fertility-related knowledge and educational experiences of U.S. medical students in their final year of school. Results: In total, 117 students (14.4%) completed the survey. The average knowledge score was 78%. Twenty-three (22%) overestimated the age of most precipitous fertility decline, and 50 (52%) overestimated the chance of getting pregnant for a 40-year-old person with ovaries. One-third of students (30, 32%) incorrectly believe that physicians have equivalent infertility rates to the general population. Students were less cognizant of male fertility issues, incorrectly believing there was no negative impact on male fertility by age (43, 42%) among other factors. Seventy-five (81%) reported less than 5 hours of fertility-related education in medical school. Only one-third (32%) were satisfied or very satisfied with the fertility education they received. In an open-response question, students expressed interest in additional education on transgender and cancer patient care, fertility preservation, assisted reproductive technologies, and reproductive life planning in medicine. Conclusions: There is a need and an opportunity for medical education programs to enhance fertility education. Giving students and trainees the knowledge required to make informed decisions for their family-building purposes and improving their ability to counsel patients adequately should be a goal of future educational endeavors. The data collected in this study will serve as a guide for the development of fertility-related learning modules for medical students and trainees.

The Formal Demography of Peak Population.

When will the human population peak? In this article, we build on classical results by Ansley Coale, who showed that when fertility declines steadily, births reach their maximum before fertility reaches replacement level, and the decline in total population size does not occur until several decades after fertility has reached that level. We extend Coale's results by modeling longevity increases, net immigration, and a slowdown in fertility decline that resembles current projections. With these extensions, our models predict a typical lag between replacement-level fertility and population decline of about 35 to 40 years, consistent with projections by the United Nations and about 15 years longer than the lag predicted by Coale. Our analysis helps reveal underlying factors in the timing of peak population.

Вопросы комплексного развития молочно-мясного скотоводства Республики Коми

The study’s relevance stems from the increase in demand for livestock products, particularly meat, juxtaposed with the growing challenges in their production on an intensive scale. These challenges include environmental degradation, climate change, and an upswing in diseases linked to imbalanced nutrition. Also, there are ongoing discussions revolving around the merit of beef and milk with normalized fat content. This study aimed to analyze the current state of dairy and beef cattle breeding in the North and formulate growth strategies aligned with the principles of sustainable development. Innovative approaches to cattle breeding have been explored, focusing on enhancing environmental, social, and economic efficiency in the industry. The "less and better" concept, centered on producing livestock products that cause minimal environmental harm, improve animal welfare, and benefit human health, has been thoroughly examined. The study also discusses the challenges confronting cattle breeding in the Komi Republic. A shift towards intensive farming has been found to boost dairy cattle productivity while also resulting in increased cattle losses and a decline in fertility and overall health. Unresolved issues related to creating an optimal feed supply system and the uniform adoption of concentrate, hay, and silage feed mixtures also contribute to industry instability. Moreover, it has been found that concerns surrounding Holsteinization and the preservation of the Kholmogorsky breed of dairy cattle are particularly acute in the region. The study’s scientific novelty lies in introducing an integrated approach to cattle breeding in the Komi Republic, aiming to improve the quality of livestock products, enhance industry profitability, and boost farmland fertility. The practical significance of the study lies in proposing measures such as establishing cultivated pastures, reintroducing cattle grazing to improve the feed supply system, and safeguarding the Kholmogorsky breed. These proposals are valuable for governing bodies involved in shaping regional strategies and programs for agricultural production development.

Testicular dysfunction and “its recovery effect” after cadmium exposure

In recent years, with the acceleration of industrialization, the decline of male fertility caused by heavy metal pollution has attracted much attention. However, whether the inhibition of testicular function after cadmium exposure is reversible remains to be studied. In this study, we constructed rat models of cadmium exposure and dis-exposure, and collected relative samples to observe the changes of related indicators. The results showed that cadmium exposure could reduce the fertility, inhibit the hypothalamic-pituitary-testis axis and activate hypothalamic-pituitary-adrenal axis function, the testicular GR/PI3K-AKT/AMPK signal was abnormal, cell proliferation was inhibited and apoptosis was enhanced. Four weeks after the exposure was stopped, the fertility was still decreased, testicular testosterone synthesis and spermatogenesis were inhibited, cell proliferation was inhibited and apoptosis was enhanced, but all of them were reversed. After eight weeks of cadmium exposure, the above indicators were observed to return to normal. At the same time, by giving different concentrations of corticosterone to spermatogonium, we confirmed that corticosterone may regulate the proliferation and apoptosis of spermatogonium through GR/PI3K-AKT/AMPK signal. In this study, the reproductive toxicity of cadmium, a metal environmental pollutant, was analyzed in depth to provide a new theoretical and experimental basis for ensuring male reproductive health.